(CMA)
(CNAS)
(ISO)
(高新技术企业)
胶原 细胞培养相关标准参考信息
GB/T 38506-2020 动物细胞培养过程中生化参数的测定方法
简介：
信息：ICS：07.080 CCS：A40 发布：2020-03-06 00:00:00.0 实施：2020-03-06 00:00:00.0
ASTM E1881-2012 用次级离子质谱法 (SIMS) 对细胞培养分析的标准指南
简介：5. Significance and UseTop Bottom 5.1 The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may also alter their chemical composition. This obstacle is overcome by using a sandwich freeze-fracture method (1). This cryogenic method has provided a unique way of sampling individual cells in their native state for SIMS analysis. 5.2 The procedure described here has been successfully used for imaging Na+ and K+ ion transport (3), calcium alterations in stimulated cells (4,5), and localization of therapeutic drugs and isotopically labeled molecules in single cells (6). The frozen freeze-dried cells prepared according to this method have been checked for SIMS matrix effects (7). Ion image quantification has also been achieved in this sample type (8). 5.3 The procedure described here is amenable to a wide variety of cell cultures and provides a way for studying the response of individual cells for chemical alterations in the state of health and disease and localization of isotopically-labeled molecules and theraputic drugs in cell culture models. 1.1 This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to complement SIMS analysis. 1.2 This guide is not suitable for cell cultures that do not attach to the substrate. 1.3 This guide is not suitable for any plastic embedded cell culture specimens. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 (Medical microbiology) CCS： 发布：2012 实施：
ASTM F813-83(1996)e1 医疗器械材料直接接触细胞培养评估标准实践
简介：
信息：ICS：07.100.10 CCS： 发布：2001-10-10 实施：
T/GDC 206—2023 细胞培养转瓶
简介：密封性、无菌性、细菌内毒素（热原）、细胞培养、容量偏差、表面处理等。
信息：ICS：83.080.01 CCS：C292 发布：2023-01-04 实施：2023-01-04
ASTM F895-11 琼脂扩散细胞培养筛选细胞毒性的标准测试方法
简介：
信息：ICS：07.100.10 CCS： 发布：2011-10-01 实施：
ASTM F813-01 医疗器械材料直接接触细胞培养评估标准实践
简介：
信息：ICS：07.100.10 CCS： 发布：2001-10-10 实施：
T/GDC 207—2023 细胞培养板
简介：外观要求、板底平整度、无菌性、表面处理、细胞毒性等。
信息：ICS：83.080.01 CCS：C292 发布：2023-01-04 实施：2023-01-04
JB/T 20137-2011 机械搅拌式动物细胞培养罐
简介：本标准规定了机械搅拌式动物细胞培养罐的术语和定义、标记、要求、试验方法、检验规则和标志、使用说明书、包装、运输、储存。本标准适用于疫苗、抗体、基因重组蛋白药物等生物制药中进行动物细胞批培养或灌流培养的机械搅拌式动物细胞培养罐(以下简称培养罐)。
信息：ICS：11.120.30 CCS：C91 发布：2011-05-18 实施：2011-08-01
NF U47-025-2001 动物健康分析方法.病毒中性试验和细胞培养免疫化学检测典型猪瘟的抗体
简介：
信息：ICS：11.220;65.020.30 CCS：B41 发布：2001-10-01 实施：2001-10-05
T/GDC 166-2022 细胞培养瓶
简介：细胞培养瓶的产品分类、要求、试验方法、检验规则及标志、包装、运输和贮存。
信息：ICS：83.080.01 CCS：C292 发布：2022-07-16 实施：2022-07-18
NF U47-035-2011 动物健康分析法.用细胞培养的病毒中和试验检测马传染性动脉炎病毒抗体
简介：
信息：ICS：11.220;65.020.30 CCS：B41 发布：2011-03-01 实施：2011-03-23
ASTM E1532-00 用双苯甲酰胺DNA结合荧光色素检测细胞培养物中支原体污染的标准实施规程
简介：
信息：ICS：07.100.10 CCS： 发布：2000-05-10 实施：
DB15/T 2555-2022 原代奶牛乳腺上皮细胞分离培养及鉴定技术规程-乳汁分离法
简介：
信息：ICS：65.020.30 CCS：B 40 发布：2022-04-25 实施：2022-05-25
ASTM F895-2011 细胞培养及毒素屏蔽的标准试验方法
简介：This test method is useful for assessing the cytotoxic potential of new materials and formulations and as part of a quality control program for established medical devices and components. This test method assumes that assessment of cytotoxicity provides useful information to aid in predicting the potential clinical applications in humans. Cell culture methods have shown good correlation with animal assays and are frequently more sensitive to cytotoxic agents. This cell culture test method is suitable for incorporation into specifications and standards for materials to be used in the construction of medical devices that are to be implanted into the human body or placed in contact with tissue fluids or blood on a long-term basis. Some biomaterials with a history of safe clinical use in medical devices are cytotoxic. This test method does not imply that all biomaterials must pass this assay to be considered safe for clinical use (Practice F748).1.1 This test method is appropriate for materials in a variety of shapes and for materials that are not necessarily sterile. This test method would be appropriate in situations in which the amount of material is limited. For example, small devices or powders could be placed on the agar and the presence of a zone of inhibition of cell growth could be examined. 1.1.1 This test method is not appropriate for leachables that do not diffuse through agar or agarose. 1.1.2 While the agar layer can act as a cushion to protect the cells from the specimen, there may be materials that are sufficiently heavy to compress the agar and prevent diffusion or to cause mechanical damage to the cells. This test method would not be appropriate for these materials. 1.2 The L-929 cell line was chosen because it has a significant history of use in assays of this type. This is not intended to imply that its use is preferred, only that the L-929 is an established cell line, well characterized and readily available, that has demonstrated reproducible results in several laboratories. 1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 (Medical microbiology) CCS：C05 发布：2011 实施：
ASTM E1531-00 用琼脂糖培养基生长检测细胞培养物中支原体污染的标准实施规程
简介：
信息：ICS：07.100.01 CCS： 发布：2000-05-10 实施：
DB15/T 2555-2022 原代奶牛乳腺上皮细胞分离培养及鉴定技术规程-乳汁分离法
简介：
信息：ICS：65.020.30 CCS：B 40 发布：2022-04-25 实施：2022-05-25
ASTM F895-2011(2016) 细胞毒性的琼脂扩散细胞培养筛选的标准试验方法
简介：4.1x00a0;This test method is useful for assessing the cytotoxic potential of new materials and formulations and as part of a quality control program for established medical devices and components. 4.2x00a0;This test method assumes that assessment of cytotoxicity provides useful information to aid in predicting the potential clinical applications in humans. Cell culture methods have shown good correlation with animal assays and are frequently more sensitive to cytotoxic agents. 4.3x00a0;This cell culture test method is suitable for incorporation into specifications and standards for materials to be used in the construction of medical devices that are to be implanted into the human body or placed in contact with tissue fluids or blood on a long-term basis. 4.4x00a0;Some biomaterials with a history of safe clinical use in medical devices are cytotoxic. This test method does not imply that all biomaterials must pass this assay to be considered safe for clinical use (Practice F748). 1.1x00a0;This test method is appropriate for materials in a variety of shapes and for materials that are not necessarily sterile. This test method would be appropriate in situations in which the amount of material is limited. For example, small devices or powders could be placed on the agar and the presence of a zone of inhibition of cell growth could be examined. 1.1.1x00a0;This test method is not appropriate for leachables that do not diffuse through agar or agarose. 1.1.2x00a0;While the agar layer can act as a cushion to protect the cells from the specimen, there may be materials that are sufficiently heavy to compress the agar and prevent diffusion or to cause mechanical damage to the cells. This test method would not be appropriate for these materials. 1.2x00a0;The L-929 cell line was chosen because it has a significant history of use in assays of this type. This is not intended to imply that its use is preferred, only that the L-929 is an established cell line, well characterized and readily available, that has demonstrated reproducible results in several laboratories. 1.3x00a0;The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4x00a0;This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 CCS： 发布：2011 实施：
JIS T0301-2000 用人工培养细胞的可植入金属的生物兼容性的试验方法
简介：この規格は，金属系ィンプラント村料の細胞適合性を，培養細胞(以下，細胞という。)を用いて評価する方法について規定する。
信息：ICS：11.060.10 CCS：C35 发布：2000-03-27 实施：
DB34/T 3987-2021 禽白血病病毒细胞培养分离检测技术规程
简介：
信息：ICS：11.220 CCS：B 41 发布：2021-09-03 实施：2021-10-03
NF U47-222-2010 通过对鱼传染性胰腺坏死病毒进行血清中和的细胞培养和鉴定的离析.
简介：
信息：ICS：11.220;65.020.30 CCS：C05 发布：2010-10-01 实施：2010-10-15
ASTM E1881-97(2002) SIMS细胞培养分析标准指南
简介：
信息：ICS：07.100.10 CCS： 发布：1997-05-10 实施：
NF T90-451-2020 试验水. 肠道病毒的检测. 玻璃棉浓缩法和细胞培养检测法
简介：Le présent document a pour objet de décrire une méthode de recherche des entérovirus dans les eaux destinées à la consommation humaine, les eaux de surface, les eaux résiduaires, et les eaux de mer sous certaines conditions et remarques définies en 6.3.1 (voir Tableau 1) .
信息：ICS：07.100.20 CCS： 发布：2020-12-11 实施：2020-12-11
NF U47-220-2010 鱼类出血性败血病病毒的荧光免疫检验法的细胞培养隔离和鉴定
简介：
信息：ICS：11.220;65.020.30 CCS：B50 发布：2010-10-01 实施：2010-10-15
ASTM E1881-97 SIMS细胞培养分析标准指南
简介：
信息：ICS： CCS： 发布：1997-05-10 实施：
ASTM E1881-12(2020) SIMS细胞培养分析标准指南
简介：
信息：ICS：07.100.10 CCS： 发布：2020-12-01 实施：
NF U47-221-2010 鱼传染性造血器官坏死病毒的免疫细胞培养和鉴定的隔离.
简介：
信息：ICS：11.220;65.020.30 CCS：B50 发布：2010-10-01 实施：2010-10-15
ASTM E1881-1997 SIMS细胞培养分析的标准指南
简介：1.1 This guide provides the Secondary Ion Mass Spectometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing into vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to compliment SIMS analysis. 1.2 This guide is not suitable for cell cultures that do not attach to the substrate. 1.3 This guide is not suitable for any plastic embedded cell culture specimens. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS： CCS：C04 发布：1997 实施：
ASTM F813-20 医疗器械材料直接接触细胞培养评估标准实践
简介：
信息：ICS：07.100.10 CCS： 发布：2020-04-01 实施：
NF U47-027-2010 动物健康分析方法.用病毒中和试验和细胞培养(IF或IP)上的免疫化学试验检测抗粘膜病抗体
简介：
信息：ICS：11.220;65.020.30 CCS：B41 发布：2010-06-01 实施：2010-06-19
ASTM E1881-1997(2002) SIMS细胞培养分析的标准指南
简介：1.1 This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to compliment SIMS analysis.1.2 This guide is not suitable for cell cultures that do not attach to the substrate.1.3 This guide is not suitable for any plastic embedded cell culture specimens.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 (Medical microbiology) CCS：C04 发布：1997 实施：
ASTM E3231-19 一次性使用材料的细胞培养生长评估的标准指南
简介：
信息：ICS： CCS： 发布：2019-10-01 实施：
NF U47-025-2010 动物健康分析方法.病毒中性试验和细胞培养免疫化学检测典型猪瘟的抗体(IF或IP)
简介：
信息：ICS：11.220;65.020.30 CCS：B41 发布：2010-06-01 实施：2010-06-19
STAS SR EN 30993-5-1996 医疗设备的生物学评价．第5部分：细胞毒性试验：在体外培养的方法
简介：
信息：ICS：11.020 CCS： 发布：1996-01-01 实施：
DB22/T 396.9-2017 保健用品毒理学评价程序与检验方法 第9部分：哺乳动物培养细胞染色体畸变试验
简介：
信息：ICS：11.020 CCS：C 04 发布：2017-11-10 实施：2017-12-30
SC 7701-2007 草鱼出血病细胞培养灭活疫苗
简介：本标准规定了草鱼出血病细胞培养灭活疫苗产品的技术要求、试验方法、检验规则、标志、包装、运输和贮存。 本标准适用于草鱼出血病细胞培养灭活疫苗。
信息：ICS：11.120.10 CCS：C27 发布：2007-12-18 实施：2008-03-01
ASTM F895-84(2001) 琼脂扩散细胞培养筛选细胞毒性的标准测试方法
简介：
信息：ICS： CCS： 发布：1995-01-01 实施：
DB 22/T 396.9-2017 保健用品毒理学评价程序与检验方法 第9部分：哺乳动物培养细胞染色体畸变试验
简介：本标准规定了保健用品毒理学哺乳动物培养细胞染色体畸变试验评价程序。本标准适用于保健用品毒理学哺乳动物培养细胞染色体畸变试验检验方法。
信息：ICS： CCS： 发布：2017-11-10 实施：2017-12-30
ASTM F813-07 医疗器械材料直接接触细胞培养评估标准实践
简介：
信息：ICS：07.100.10 CCS： 发布：2007-02-01 实施：
ASTM F895-84(2001)e1 琼脂扩散细胞培养筛选细胞毒性的标准测试方法
简介：
信息：ICS：07.100.10 CCS： 发布：1995-01-01 实施：
176兽药典 三部-2015 附录目次 试剂、试液、培养基 3706 细胞培养用营养液及溶液的配制法
简介：无
信息：ICS： CCS： 发布：2016-08-23 实施：2016-11-15
ASTM F813-2007(2012) 医疗器械材料直接接触细胞培养评价的标准实施规程
简介：4. Significance and UseTop Bottom 4.1 This practice is useful for assessing cytotoxic potential both when evaluating new materials or formulations for possible use in medical applications, and as part of a quality control program for established medical materials and medical devices. 4.2 This practice assumes that assessment of cytotoxicity potential provides one method for predicting the potential for cytotoxic or necrotic reactions to medical materials and devices during clinical applications to humans. In general, cell culture testing methods have shown good correlation with animal assays and are frequently more sensitive to toxic moieties. 4.3 This cell culture test method is suitable for adoption in specifications and standards for materials for use in the construction of medical devices that are intended to be implanted in the human body or placed in contact with tissue, tissue fluids, or blood on a long-term basis. However, care should be taken when testing materials that are resorbable to be sure the method is applicable. 4.4 Since cells in this direct contact test method are not protected by an overlying agarose layer, they are more susceptible to potential mechanical damage imparted by the overlying test sample. Investigators wishing to evaluate the cytotoxic response of cells underlying the test sample should consider agarose-based methods similar to Test Method F895. Alternatively, depending on sample characteristics, extraction methods such as Practice F619 may also be considered. 1.1 This practice covers a reference method of direct contact cell culture testing which may be used in evaluating the cytotoxic potential of materials for use in the construction of medical materials and devices. 1.2 This practice may be used either directly to evaluate materials or as a reference against which other cytotoxicity test methods may be compared. 1.3 This is one of a series of reference test methods for the assessment of cytotoxic potential, employing different techniques. 1.4 Assessment of cytotoxicity is one of several tests employed in determining the biological response to a material, as recommended in Practice F748. 1.5 The L-929 cell line was chosen because it has a significant history of use in assays of this type. This is not intended to imply that its use is preferred; only that the L-929 is a well-characterized, readily available, established cell line that has demonstrated reproducible results in several laboratories. 1.6 Since the test sample is not removed at the time of microscopic evaluation and underlying c......
信息：ICS：07.100.10 (Medical microbiology) CCS：C30 发布：2007 实施：
NF X42-052-1989 生物工程.制定获自细胞培养物的单克隆抗体的工业生产的良好实用方法指南
简介：
信息：ICS：07.080 CCS：A40 发布：1989-12 实施：1989-11-05
ASTM F895-11(2016) 琼脂扩散细胞培养筛选细胞毒性的标准测试方法
简介：
信息：ICS：07.100.10 CCS： 发布：2016-04-01 实施：
ASTM F813-2007 直接接触细胞培养评估医疗器械材料的标准实施规程
简介：This practice is useful for assessing cytotoxic potential both when evaluating new materials or formulations for possible use in medical applications, and as part of a quality control program for established medical materials and medical devices. This practice assumes that assessment of cytotoxicity potential provides one method for predicting the potential for cytotoxic or necrotic reactions to medical materials and devices during clinical applications to humans. In general, cell culture testing methods have shown good correlation with animal assays and are frequently more sensitive to toxic moieties. This cell culture test method is suitable for adoption in specifications and standards for materials for use in the construction of medical devices that are intended to be implanted in the human body or placed in contact with tissue, tissue fluids, or blood on a long-term basis. However, care should be taken when testing materials that are resorbable to be sure the method is applicable. Since cells in this direct contact test method are not protected by an overlying agarose layer, they are more susceptible to potential mechanical damage imparted by the overlying test sample. Investigators wishing to evaluate the cytotoxic response of cells underlying the test sample should consider agarose-based methods similar to Test Method F 895. Alternatively, dependent on sample characteristics, extraction methods such as Practice F 619 may also be considered.1.1 This practice covers a reference method of direct contact cell culture testing which may be used in evaluating the cytotoxic potential of materials for use in the construction of medical materials and devices.1.2 This practice may be used either directly to evaluate materials or as a reference against which other cytotoxicity test methods may be compared.1.3 This is one of a series of reference test methods for the assessment of cytotoxic potential, employing different techniques.1.4 Assessment of cytotoxicity is one of several tests employed in determining the biological response to a material, as recommended in Practice F 748.1.5 The L-929 cell line was chosen because it has a significant history of use in assays of this type. This is not intended to imply that its use is preferred; only that the L-929 is a well-characterized, readily available, established cell line that has demonstrated reproducible results in several laboratories.1.6 Since the test sample is not removed at the time of microscopic evaluation and underlying cells may be affected by the specific gravity of the test sample, this practice is limited to evaluation of cells outside the perimeter of the overlying test sample.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 (Medical microbiology) CCS：C30 发布：2007 实施：
ASTM F895-1984(2006) 琼脂扩散细胞培养屏蔽细胞毒素的试验方法
简介：This test method is useful for assessing the cytotoxic potential of new materials and formulations and as part of a quality control program for established medical devices and components. This test method assumes that assessment of cytotoxicity provides useful information to aid in predicting the potential clinical applications in humans. Cell culture methods have shown good correlation with animal assays and are frequently more sensitive to cytotoxic agents. This cell culture test method is suitable for incorporation into specifications and standards for materials to be used in the construction of medical devices that are to be implanted into the human body or placed in contact with tissue fluids or blood on a long-term basis. Some biomaterials with a history of safe clinical use in medical devices are cytotoxic. This test method does not imply that all biomaterials must pass this assay to be considered safe for clinical use (Practice F 748).1.1 This test method is appropriate for materials in a variety of shapes and for materials which are not necessarily sterile. This test method would be appropriate in situations where the amount of material is limited. For example, small devices or powders could be placed on the agar and the presence of a zone of inhibition of cell growth could be examined. 1.1.1 This test method is not appropriate for leachables which do not diffuse through agar or agarose. 1.1.2 While the agar layer can act as a cushion to protect the cells from the specimen, there may be materials which are sufficiently heavy to compress the agar and prevent diffusion or to cause mechanical damage to the cells. This test method would not be appropriate for these materials. 1.2 The L-929 cell line was chosen because it has a significant history of use in assays of this type. This is not intended to imply that its use is preferred, only that the L-929 is an established cell line, well-characterized and readily available, that has demonstrated reproducible results in several laboratories. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 (Medical microbiology) CCS：C05 发布：1984 实施：
SN/T 3899-2014 化妆品体外替代试验良好细胞培养和样品制备规范
简介：本标准规定了化妆品体外替代试验应遵守的良好细胞培养规范和样品制备规范。本标准适用于化妆品原料及产品体外毒理学试验细胞培养实验室。如适用,也可用于药物、食品及添加剂、日用化学品、化工原料和产品体外毒理学试验实验室参考。
信息：ICS： CCS： 发布：2014-01-13 实施：2014-08-01
ASTM E1881-06 SIMS细胞培养分析标准指南
简介：
信息：ICS：07.100.10 CCS： 发布：2006-11-01 实施：
简介： 信息：
ASTM F2997-2013 采用荧光图像分析量化祖细胞成骨培养钙沉积的标准实施规程
简介：5.1x00a0;In-vitro osteoblast differentiation assays are one approach to screen progenitor stem cells for their capability to become osteoblasts. The extent of calcified deposits or mineralized matrix that form in-vitro may be an indicator of differentiation to a functional osteoblast; however, gene expression of osteogenic genes or proteins is another important measurement to use in conjunction with this assay to determine the presence of an osteoblast. 5.2x00a0;This test method provides a technique for staining, imaging, and quantifying the fluorescence intensity and area related to the mineralization in living cell cultures using the non-toxic calcium-chelating dye, xylenol orange. The positively stained area of mineralized deposits in cell cultures is an indirect measure of calcium content. It is important to measure the intensity to assure that the images have not been underexposed or overexposed. Intensity does not correlate directly to calcium content as well as area. 5.3x00a0;Xylenol orange enables the monitoring of calcified deposits repeatedly throughout the life of the culture without detriment to the culture. There is no interference on subsequent measurements of mineralized area due to dye accumulation from repeated application (1).3 Calcified deposits that have been previously stained may appear brighter, but this does not impact the area measurement. Calcein dyes may also be used for this purpose (1) but require a different procedure for analysis than xylenol orange (i.e., concentration and filter sets) and are thus not included here. Alizarin Red and Von Kossa are not suitable for use with this procedure on living cultures since there is no documentation supporting their repeated use in living cultures without deleterious effects. 5.4x00a0;The test method may be applied to cultures of any cells capable of producing calcified deposits. It may also be used to document the absence of mineral in cultures where the goal is to avoid mineralization. 5.5x00a0;During osteoblast differentiation assays, osteogenic supplements are provided to induce or assist with the differentiation process. If osteogenic supplements are used in excess, a calcified deposit may occur in the cell cultures that is not osteoblast-mediated and thus is referred to as dystrophic, pathologic, or artifactual (2). For example, when higher concentrations of beta-glycerophosphate are used in the medium to function as a substrate for the enzyme alkaline phosphatase secreted by the cells, there is a marked increase in free phosphate, which then precipitates with Ca++ ions in the media to form calcium phosphate crystals independently of the differentiation status of the progenitor cell. Alkaline phosphatase production is associated with progenitor cell differentiation, and is frequently stimulated by dexamethasone addition to the medium, which enhances the formation of calcified deposits. These kinds of calcified/mineral deposits are thus considered dystrophic, pathologic, or artifactual because they were not initiated by a mature osteoblast. The measurement obtained by using this practice may thus result in a potentially false interpretation of the differentiation status of osteoprogenitor cells if used in isolation without gene or protein expression data (3,4). 5.6x00a............
信息：ICS：07.100.10 (Medical microbiology) CCS： 发布：2013 实施：
ASTM F895-84(2006) 琼脂扩散细胞培养筛选细胞毒性的标准测试方法
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信息：ICS：07.100.10 CCS： 发布：2006-03-01 实施：
简介： 信息：
ASTM E1881-12 SIMS细胞培养分析标准指南
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信息：ICS：07.100.10 CCS： 发布：2012-11-01 实施：
ASTM E1881-2006 用次级离子质谱法(SIMS)进行细胞培养分析的标准指南
简介：The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may also alter their chemical composition. This obstacle is overcome by using a sandwich freeze-fracture method (1). This cryogenic method has provided a unique way of sampling individual cells in their native state for SIMS analysis. The procedure described here has been successfully used for imaging Na+ and K+ ion transport (3), calcium alterations in stimulated cells (4,5), and localization of therapeutic drugs and isotopically labeled molecules in single cells (6). The frozen freeze-dried cells prepared according to this method have been checked for SIMS matrix effects (7). Ion image quantification has also been achieved in this sample type (8). The procedure described here is amenable to a wide variety of cell cultures and provides a way for studying the response of individual cells for chemical alterations in the state of health and disease and localization of isotopically-labeled molecules and theraputic drugs in cell culture models.1.1 This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to complement SIMS analysis.1.2 This guide is not suitable for cell cultures that do not attach to the substrate.1.3 This guide is not suitable for any plastic embedded cell culture specimens.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
信息：ICS：07.100.10 (Medical microbiology) CCS：C04;A40 发布：2006 实施：
简介： 信息：
ASTM F813-07(2012) 医疗器械材料直接接触细胞培养评估标准实践
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信息：ICS：07.100.10 CCS： 发布：2012-10-01 实施：
NF U47-200-2004 动物健康分析方法.细胞培养的实用指南
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信息：ICS：65.020.30;11.220 CCS：B41 发布：2004-12-01 实施：2004-12-20
简介： 信息：
NY/T 2186.4-2012 微生物农药毒理学试验准则.第4部分：细胞培养试验
简介：本部分规定了细胞培养试验的基本原则、方法和要求。本部分适用于为微生物农药登记而进行的细胞培养试验。
信息：ICS：65.020.01 CCS：B17 发布：2012-06-06 实施：2012-09-01
NF U47-027-2002 动物健康分析方法.用病毒中性试验和在细胞上培养(IF或IP)免疫化学试验检测抗粘膜传染病抗体
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信息：ICS：11.220;65.020.30 CCS：B41 发布：2002-06-01 实施：2002-06-05
简介： 信息：
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